Welcome to Lab Test Consult

You can log in and post your comments

Share your knowledge and experience!

Member Login
Lost your password?

What is HPLC?


HPLC is High Performance Liquid Chromatography.

Russian botanist Tswett first discovered Liquid Chromatography (LC) in the 1800s, testing to separate and isolate various plant pigments.

He used the term chromatography (color writing) because of the colored bands produced on the adsorbent bed when he washed the column that hold stationary phase with solvent (mobile phase) under the aid of gravity.

To get better separation, densely packed columns and pumps (rather than gravity) provides the higher pressure required to move the mobile phase and analyte through the densely packed column were used in 1970s.  HPLC was coined by Prof. Csaba Horváth for his 1970 Pittcon paper, originally indicated the fact that high pressure was used to generate the flow required for liquid chromatography in packed columns.

HPLC really began to take hold in the late 1970s. With continued advances in performance during this time, the acronym HPLC remained the same, but the name was changed to high performance liquid chromatography.

In principle, LC and HPLC work the same way except the speed, efficiency, sensitivity and ease of operation of HPLC is vastly superior.

HPLC is now one of the most powerful tools in analytical chemistry, with the ability to separate, identify and quantitate the compounds that are present in any sample that can be dissolved in a liquid, such as pharmaceuticals, clinical samples, food, nutraceuticals, cosmetics, environmental matrices and industry chemicals, etc.

The basic components of an HPLC system include

Solvent reservoir

Sample injector

Pump(s)

Column

Detector(s)

Data collector and control system

=======================================================================

HPLC System Operation

HPLC Troubleshooting

=======================================================================

 

Share

Leave a Reply



New post

Posts

November 2017
M T W T F S S
« Aug    
 12345
6789101112
13141516171819
20212223242526
27282930  

Posts

Recent comments

    Contact Sign In

    Email Marketing You Can Trust